They are often unrelated. All characters have unique qualities--some good, some bad--that influence how they behave. The story's goal may follow a particular theme. Occasionally a story can have multiple themes if there are interweaving subplots. At times, their personality traits are central to the theme--always telling the truth. They are not opposite or the same. They are often in parallel and complementary.
Theme: Traveling from Madrid to Moscow
Plot: specific route to get there
Genre: mode of transportation if restricted.
Character: types of vehicles/transportation with pro's and con's
"Acts": stop-offs for sightseeing and excursions while traveling
Just my quick thoughts.